The Preparation of Apigenin Nanoparticles and the Study of Their Anti-Inflammatory and Anti-Tumor Activities In Vitro

Apigenin (API) has many biological activities, but its poor solubility limits clinical application. In this research, API nanoparticles were prepared by the liquid antisolvent precipitation (LAP) technique, which effectively improved and bioavailability of API. Through design a single-factor test, effects type dosage surfactants, concentration, to solvent volume ratio, speed time stirring, temperature precipitation, dropping on MPS (mean particle size) nanosuspension carried out. The optimum technological conditions determined as follows: 5 mg/mL tween 80 surfactant, 20 API, an antisolvent/solvent ratio 10, 1200 r/min stirring for min, 45 °C temperature, 1 mL/min speed. Under conditions, we obtained with 170.5 nm then it was freeze-dried obtain nanoparticles. Moreover, characterized SEM, FTIR, XRD, DSC, TG. Results showed that although transformed into amorphous form, their internal chemical structure had not been changed higher solubility. Finally, nanoparticles’ anti-inflammatory activities evaluated observing effect nitric oxide (NO) production IL-10 toward RAW264.7 cells induced lipopolysaccharide (LPS). anti-tumor testing cell viability apoptosis. results suggested exhibited much better compared raw